Identification of Ergosterol in Mushrooms
نویسندگان
چکیده
Mushroom has gained popularity as prized table delicacy with high nutritive and medicinal value. Ergosterol is a biological precursor (a provitamin) to vitamin D2. The contents of vitamin D2 and sterols in some wild and cultivated mushrooms were determined. Vitamin D2 was determined using an HPLC method, including saponification and semipreparative normalphase HPLC purification Lv et al. [1]. Currently HPLC methods are regularly practiced for the estimation / determination of ergosterol [2]. The original procedure for extraction of ergosterol was worked out by Seitz et al. [3]. Four mushrooms Calocybe indica, Ganoderma lucidum, Pleurotus florida and Volvariella volvacea were grown on two synthetic (complete yeast extract agar and Lambert’s agar) media and three semisynthetic (malt extract agar, rice bran decoction and and wheat extract agar) media. The mycelial biomass of each mushroom was subjected to extraction of ergosterol and its identification using high performance liquid chromatography (HPLC). The ergosterol content ranged from 113 to 403μg/ g with lowest retention peak was observed in P. florida showing 113 μg ergosterol per gram where as C. indica showed 243 μg ergosterol per gram and V. volvacea shows 159 μg ergosterol per gram and highest retention peak was observed in G. lucidum showing 403 μg ergosterol per gram of sample.
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